Neuronal Differentiation of NT2 Cells in Monolayer and Spheroid Cultures

Metabolism and differentiation of cultured cells are influenced by changes in cellular morphology. In this study, we investigated the differences cell proliferation neuronal NT2 monolayer (2D) spheroid (3D) cultures. culture, adhered extended on a tissue culture plate. For fabricated microwell chip comprising 195 circular microwells (600 ìm diameter) cutture plate, surface was modified with polyethylene glycol to promote formation. The were aggregated each formed spheroids within 24 hours morphology maintained thoughout period. Although ability higher than that culture. Furthermore, dramatically enhanced retinoic acid treatment. These results indicate properties morphologies, is promising technique induce differentiation.